JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
高通量酵母质粒过表达屏幕
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
High-throughput Yeast Plasmid Overexpression Screen
Please note that all translations are automatically generated.
Click here for the English version.
高通量酵母质粒过表达屏幕
DOI:
10.3791/2836-v
•
08:57 min
•
July 27, 2011
•
Michael S. Fleming
,
Aaron D. Gitler
1
Neuroscience Graduate Group
,
University of Pennsylvania School of Medicine
,
2
Department of Cell and Developmental Biology
,
University of Pennsylvania School of Medicine
Chapters
00:05
Title
00:56
Preparations for Yeast Transformation
02:19
Yeast Transformation Using a Liquid Handling Robot
06:04
Spotting Assay
07:10
Representative Results: Plasmid Overexpression Screen
08:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们描述了一个质粒的过度表达屏幕<em>酿酒酵母</em>,使用液体处理机器人摆着质粒库和高通量酵母转化协议。
Tags
High-throughput
Yeast
Plasmid
Overexpression Screen
Saccharomyces Cerevisiae
Genetic Screens
Gene Function
Liquid Handling Robot
Arrayed Library
Neurodegenerative Disease Proteins
Cellular Phenotypes
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一个Biomining从宏基因组库的纤维素酶的活性高通量筛选
木质纤维素材料的高通量检测糖化
高通量的亲和标签的重组蛋白的纯化
使用高通量形状的RNA二级结构预测
海胆受精卵的高通量显微注射
萤光素酶表达的重组病毒的高通量滴定
手册小分子屏幕接近高通量使用斑马鱼胚胎
恢复成年斑马鱼的心脏的高通量应用
高通量筛选转录后调控基因的化学调节剂
高通量<em>斑马鱼</em>能量消耗分析
Read Article