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Chapters
- 00:05Title
- 00:09Introduction
- 02:56Detection of Nitric Oxide (NO) with Culture of Bovine Aortic Endothelial Cells (BAEC)
- 05:56Endothelial NOS (eNOS) Uncoupling Experiment using SIN-1
- 08:12Detection of superoxide (O2–) from polymorphonuclear neutrophils (PMNs)
- 10:20Conclusion
Résonance paramagnétique électronique (RPE) a été utilisé pour détecter l'oxyde nitrique à partir de cellules endothéliales aortiques bovines et de l'anion superoxyde radical de neutrophiles humains en utilisant le fer (II)-N-méthyl-D-glucamine dithiocarbamate, Fe (MGD)<sub> 2</sub> Et de 5,5-diméthyl-1-pyroroline-N-oxyde, DMPO, respectivement.
Tags
DetectionNitric OxideSuperoxide Radical AnionElectron Paramagnetic Resonance SpectroscopyCellsSpin TrapsReactive Nitrogen SpeciesReactive Oxygen SpeciesCell FunctionImmune ResponseAntioxidant Defense MechanismsOxidative DamageProtein FunctionDNA CleavageLipid PeroxidationOxidative StressCell InjuryCell DeathSuperoxide Radical Anion GenerationPeroxynitriteHydroxyl RadicalPolymorphonuclear NeutrophilsChemotactic StimulationPhorbol-12-myristate-13-acetateNicotinamide Adenine Dinucleotide Phosphate OxidaseNitric Oxide Synthase
