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染色质免疫沉淀从以下轴索损伤的背根神经节组织
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JoVE Journal
Neuroscience
Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
Please note that all translations are automatically generated.
Click here for the English version.
染色质免疫沉淀从以下轴索损伤的背根神经节组织
DOI:
10.3791/2803-v
•
09:41 min
•
July 20, 2011
•
Elisa Floriddia*
1,2
,
Tuan Nguyen*
1
,
Simone Di Giovanni
1
Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research
,
University of Tuebingen
,
2
Graduate School for Cellular and Molecular Neuroscience
,
University of Tuebingen
Chapters
00:05
Title
01:04
Sciatic & Dorsal Column Nerve Injury
04:35
Cross-linking
06:14
Immunoprecipitation and Elution
08:23
Increased Expression After Nerve Injury
09:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们目前从背根神经节组织中的染色质免疫沉淀性轴索损伤的方法。这种方法可以用来识别特定的转录因子结合位点,同时在周边及中枢神经系统受伤的轴突再生的表观遗传修饰的组蛋白与DNA的重要。
Tags
Chromatin Immunoprecipitation
Dorsal Root Ganglia
Axonal Injury
Central Nervous System
Peripheral Nervous System
Neurite Outgrowth
Axonal Regeneration
Transcriptional Programs
Gene Regulation
Injury Model System
Transcriptional Activity
Animal Removal
Nerve Fibers
Sciatic Nerve
Spinal Cord
Transcription Factor Binding
Histone Modification
DNA Modification
ChIP Protocol
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