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Journal
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Biology
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全山
原位
杂交E8.5,E11.5小鼠胚胎
JoVE Journal
Biology
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JoVE Journal
Biology
Whole Mount
in Situ
Hybridization of E8.5 to E11.5 Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
全山
原位
杂交E8.5,E11.5小鼠胚胎
DOI:
10.3791/2797-v
•
13:54 min
•
October 10, 2011
•
Qiaozhi Wei
,
Nancy R. Manley
,
Brian G. Condie
1
Department of Genetics
,
University of Georgia
Chapters
00:05
Title
01:09
Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
03:58
Collection of Mouse Embryos
05:12
Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
10:07
Antibody Detection of the Digoxygenin-labeled Riboprobe
12:38
Examples of Whole-mount
in situ
Hybridizations
13:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这整个坐骑<em>原位</em>杂交协议的讨论,以确保在E8.5 - E11.5日龄小鼠胚胎的基因表达研究的重复性高品质的成果的关键步骤。
Tags
Whole Mount
In Situ Hybridization
Gene Expression Patterns
Embryos
Probe Labeling
Quality Control
DIG-labeled RNA Probes
In Vitro Transcription
PCR
Sensitivity
Endogenous MRNA
Fixation
Storage
Proteinase K Digestion
Hybridization Conditions
Post-hybridization Washes
RNase Treatment
Non-specific Probe Hybridization
AP-conjugated Antibody
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