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用于测量高分子配体亲和等温滴定量热
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
Please note that all translations are automatically generated.
Click here for the English version.
用于测量高分子配体亲和等温滴定量热
DOI:
10.3791/2796-v
•
08:45 min
•
September 07, 2011
•
Michael R. Duff, Jr.
,
Jordan Grubbs
,
Elizabeth E. Howell
1
Department of Biochemistry, Cellular and Molecular Biology
,
University of Tennessee
Chapters
00:05
Title
01:06
Isothermal Titration Calorimetry (ITC) Sample Preparation
02:34
The ITC Experiment
05:46
ITC Data Analysis
07:20
Representative ITC Results
08:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
使用温和的亲和力,以监测生物系统的结合热力学等温滴定量热的一个协议。
Tags
Isothermal Titration Calorimetry
ITC
Macromolecule-ligand Affinity
Binding Reaction
Thermodynamic Picture
Biological Sciences
Macromolecular Interactions
Experimental Conditions
Buffer
Temperature
Ligand Concentration
Macromolecule Concentration
Accurate Determination
Sample Preparation
Impurities
ITC Experiment Controls
Stoichiometry
Affinity
Enthalpy
Additional Experiments
Buffer Conditions
Temperature Conditions
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