A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 02:00Transfection of Primary Cultured Cortical Neurons
- 03:14Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips
- 03:46Fixation and Immunocytohistochemistry
- 05:21Quantitative Analysis of Spine Morphologies
- 06:58Time-lapse Imaging
- 09:43Representative Results: Analysis of Dendritic Spine Morphology
- 11:04Conclusion
Numerosos estudos recentes identificaram mutações em proteínas sinápticas associada a patologias cerebrais. Primários cultivados neurônios corticais oferecem grande flexibilidade para examinar os efeitos destas proteínas doenças associadas na morfologia e motilidade espinha dendrítica.
Tags
Dendritic SpinesMorphologyCultured NeuronsExcitatory ConnectionsSynapsesActinHebbian PlasticityNeuromodulatory SignalsNeural CircuitsInformation ProcessingSynaptic ProteinsDisease StatesSchizophreniaAutism Spectrum DisordersAberrant Spine PlasticityPathophysiologyCultured Cortical NeuronsHigh-resolution ImagingFixed CellsTime-lapse Imaging
