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Journal
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Immunology and Infection
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到哺乳动物的上皮
细胞在
体外细菌粘附的含量
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
In Vitro
Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
到哺乳动物的上皮
细胞在
体外细菌粘附的含量
DOI:
10.3791/2783-v
•
05:57 min
•
May 16, 2011
•
Jason Letourneau
,
Cynthia Levesque
,
Frederic Berthiaume
,
Mario Jacques
,
Michael Mourez
1
Universite de Montreal
,
Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Faculte de medecine veterinaire
Chapters
00:05
Title
00:58
Prepare Bacterial Strains and Epithelial Cells
02:34
Infection of Cells
04:15
Representative cfu Counting Results
05:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此协议是一个简单的细菌粘附实验,计数菌落形成单位,坚持到培养细胞的数量。该检测是可靠的,独立的黏附研究,并有众多的变化在大多数实验室中使用细菌发病机制的工作。
Tags
In Vitro Assay
Bacterial Adhesion
Mammalian Epithelial Cells
Bacterial Pathogens
Adhesins
Host Cell Surface Receptors
Cellular Microbiology
Bacterial Pathogenesis Studies
Adherence Assay
Escherichia Coli Strain 27875
Autotransporter Adhesin Involved In Diffuse Adherence (AIDA)
AidA Gene
HEp-2 Human Epithelial Cell Line
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