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Immunology and Infection
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一种简单而有效的隔离混合原代培养成人肝细胞巨噬细胞的方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种简单而有效的隔离混合原代培养成人肝细胞巨噬细胞的方法
DOI:
10.3791/2757-v
•
08:10 min
•
May 24, 2011
•
Hiroshi Kitani
,
Takato Takenouchi
,
Mitsuru Sato
,
Miyako Yoshioka
,
Noriko Yamanaka
1
Transgenic Animal Research Center
,
National Institute of Agrobiological Sciences, Tsukuba, Japan
,
2
Safety Research Team
,
National Institute of Animal Health, Tsukuba, Japan
Chapters
00:05
Title
02:14
Perfusion of the Liver
03:33
Preparation and Culture of Parenchymal Hypatocyte-rich Cell Fraction
04:36
Selective Isolation of Macrophages
06:46
Results: Highly-purified Rat Liver Macrophages
07:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
获得原代培养大鼠肝细胞的巨噬细胞的一个新的方法来描述。这种方法利用在文化的巨噬细胞增殖,选择性附件晃动培养瓶和塑料菜的净化。这项技术有效地提供肝巨噬细胞,而无需复杂的设备和技能。
Tags
Isolate Macrophages
Mixed Primary Cultures
Adult Liver Cells
Kupffer Cells
Liver-specific Resident Macrophages
Isolation Methods
Sophisticated Equipment
Centrifugal Elutriator
Technical Skills
Novel Method
Sufficient Number And Purity
Rat Liver Cells
Two-step Perfusion Method
Parenchymal Hepatocytes
Culture Medium
DMEM
FCS
Epithelial Cell Morphology
Fibroblast-like Cells
Phase Contrast-bright Round Macrophage-like Cells
Proliferation Of Macrophage-like Cells
Shaking Of Culture Flasks
Suspension Of Macrophages
Transfer And Short Incubation In Plastic Dishes
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