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高通量筛选和生物传感与荧光 C。线虫菌株
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JoVE Journal Biology
High-throughput Screening and Biosensing with Fluorescent C. elegans Strains
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高通量筛选和生物传感与荧光 C。线虫菌株

DOI:
10.3791/2745-v

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14:53 min

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May 19, 2011

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, , ,
1Department of Biology,University of Florida, 2Mount Desert Island Biological Laboratory

Chapters

  • 00:05Title
  • 01:42Preparation of Bacterial Worm Food
  • 03:04Large-scale C. elegans Liquid Culture
  • 07:39Worm Collection and Preparation
  • 10:46Fluorescence Analysis
  • 11:30Representative Results
  • 13:51Conclusion

Summary

Automatic Translation

Automatic Translation

液为基础的培养和分配过程<em> C。线虫</em>菌株表达荧光记者蛋白质的描述，不需要昂贵的分拣设备。这种方法可以适用于众多诱导<em> C。线虫</em>基因药物发现或污染物的生物传感。

Tags

High-throughput ScreeningBiosensingFluorescent C. Elegans StrainsChemical ModulatorsBiological ProcessesCell Culture ModelsIn Vivo ScreeningWhole Animal ModelsDrug DevelopmentC. Elegans As A Model OrganismDrug Mode-of-action IdentificationCulturing And Dispensing ProtocolHigh-throughput Screening Of Chemical LibrariesDetection Of Environmental ContaminantsGene Expression AlterationFluorescence Microplate Reader

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