A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:26Expression of the Chimeric Proteins
- 02:30Measurement of β-Galactosidase Activity
- 03:40Verification of Protein Expression
- 04:24Control for Proper Membrane Insertion
- 05:34Representative Results of the ToxR Transcriptional Reporter Assay
- 06:17Conclusion
Tags
Transmembrane DomainOligomerization PropensityToxR AssayProtein Transmembrane DomainsPhospholipid BilayersMembrane-spanning ProteinsHydrophobic HelicesStructured Transmembrane OligomersAmino AcidsMembrane EnvironmentAqueous SolutionIntermolecular ForcesProtein AssociationMolecular RecognitionWater-soluble RegionsTransmembrane OligomerizationAssociation MeasurementIntegral Membrane Protein FunctionSynthetic PeptidesCellular Membranes
