A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
- 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
- 06:33Preparation of Collagen Embedded Cells for Grafting
- 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
- 10:40Representative Results
Dans ce rapport, nous démontrons un système pour isoler les cellules du donneur et de la culture de la glande mammaire de la souris, et la transplantation orthotopique ces cellules dans des souris bénéficiaires d'analyser stromales: interactions épithéliales pendant le développement de tumeurs mammaires.
Tags
Mammary TransplantationStromal CellsCarcinoma CellsC57BL/6J MiceTumor ProgressionMouse ModelsEpithelial CellsImmunocompromised MiceExtracellular MatricesMatrigelRat Tail CollagenFunctional T CellsDrug DevelopmentEfficacyOrthotopic TransplantationCarcinoma Associated FibroblastsPyVmT Mammary Carcinoma CellsCollagenInguinal Mammary Glands
