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Immunology and Infection
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星状细胞的七个步骤
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Seven Steps to Stellate Cells
Please note that all translations are automatically generated.
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星状细胞的七个步骤
DOI:
10.3791/2710-v
•
06:40 min
•
May 10, 2011
•
Patrick Maschmeyer
,
Melanie Flach
,
Florian Winau
1
Immune Disease Institute, Program in Cellular and Molecular Medicine at Children’s Hospital, Department of Pathology
,
Harvard Medical School
Chapters
00:05
Title
00:45
In Situ Perfusion of Mouse Livers with Digestive Enzymes
02:37
In Vitro Digestion of Mouse Livers
03:10
Density Gradient Centrifugation
05:29
Results of Isolating Hepatic Stellate Cells
06:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了从小鼠肝的肝星状细胞的分离方法。小鼠肝脏星状细胞的纯化,消化<em>原位</em><em>在体外</em>链霉蛋白酶,胶原酶治疗之前,密度梯度离心法。这种技术产生高纯度的肝星状细胞。
Tags
Stellate Cells
Hepatic Stellate Cells
Liver-resident Cells
Space Of Disse
Bone Marrow Precursors
Vitamin A Storage
Myofibroblasts
Extracellular Matrix
Liver Fibrosis
Portal Hypertension
Antigen Presenting Cells
NKT Cells
T Lymphocytes
Isolation Of Stellate Cells
Perisinusoidal Localization
Digestive Enzymes
Pronase E
Collagenase P
In Vitro Enzymatic Treatment
Vitamin A-storing Lipid Droplets
Centrifugation
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