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Chapters
- 00:05Title
- 01:59Injection of mRNA Encoding the Fluorescently-tagged Actin Binding Protein
- 03:47Induction of Cell Extrusion in Zebrafish Larvae Using G418
- 04:47Mounting Zebrafish Larvae for Imaging
- 05:34Live Imaging of Actin Dynamics and Individual Epithelial Cell Behaviors during Cell Extrusion Using a Spinning Disc Confocal Microscope
- 08:22Representative Epidermal Images
- 08:55Conclusion
Dying hücreler komşu hücrelerle birlikte daralma bariyer fonksiyonunu bozmadan epitel dokuların ekstrüde. Gelişmekte zebrafish optik netlik epitel yaşam ekstrüzyon görselleştirmek için mükemmel bir sistem sağlar. Burada hücresel çözünürlükte larva zebrafish epidermisin neden ve görüntü ekstrüzyon yöntemleri açıklanmaktadır.
Tags
Live ImagingCell ExtrusionEpidermisDeveloping ZebrafishDamaged CellsBarrier FunctionDying CellsActin And MyosinEpithelial SheetCell Extrusion ProcessOptical ClarityLarval Zebrafish EpidermisFluorescent Protein Labeled ProbeF-actinTransgenic Zebrafish EmbryosGreen Fluorescent ProteinApoptosisG418Time-lapse ImagingSpinning Disc Confocal MicroscopeActin DynamicsEpithelial Cell BehaviorsApoptotic Cell Elimination
