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Chapters
- 00:05Title
- 01:09Agrobacterium Transient Infiltration
- 04:04Assessing Results with a Histochemical GUS Assay
- 04:50Assessing Results with a Fluorometric MUG Assay
- 06:34Cell Death as Measured by GUS Expression
- 07:04Conclusion
Programlanmış hücre ölümü testleri gibi DNA laddering veya TUNEL deneyleri, memeli sistemlerinde yaygın olarak kullanılan bitkiler üretmek için zor. GUS muhabiri sistemi ile birlikte, biz, belirli genlerin potansiyel ölüm özelliklerini analiz etmek için hızlı bir şekilde, bitki bazlı geçici tahlil öneriyoruz.
Tags
A β-glucuronidase (GUS)Transient Plant Expression SystemReporter GenePositive Regulators Of Cell DeathN. Benthamiana LeavesCo-infiltration35S Driven Expression CassetteGene AnalysisGUS Vector PCAMBIA 2301Agrobacterium Strain LBA4404Live CellsMarker GeneAnti-apoptotic GenesPro- And Anti-death PlayersBcl-2 Associated AthanoGene (BAG) FamilyMammalian Inducers Of Cell DeathBAXPost-translational Modification/activation Of ProteinsRapid And Sensitive Plant Based Method
