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免疫组化:人类神经干细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Immunocytochemistry: Human Neural Stem Cells
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免疫组化:人类神经干细胞
DOI:
10.3791/267-v
•
19:41 min
•
August 24, 2007
•
Steven Marchenko
,
Lisa Flanagan
1
Department of Pathology
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:07
Introduction
00:57
Day 1: Preparing German Glass Coverslips and Seeding Cells
03:16
Day 2: Fixing, Permeablilization, and Adding Primary Ab to Cells
12:47
Day 3: Washing, secondary Ab incubation, nuclear stain, and mountin
18:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
免疫细胞化学是一个功能强大的方法来确定是否存在,亚细胞定位和兴趣在培养的细胞抗原的相对丰度。该协议提出了一个易于遵循的一系列步骤,使一个保护抗体染色。
Tags
Immunocytochemistry
Human Neural Stem Cells
Antigen
Protein
Cultured Cells
Subcellular Localization
Relative Abundance
Antibodies
Staining
Protocol
Qualitative Data
Quantitative Data
Coverslips
Antibody Solution
Parafilm
Sample
Immunostaining
HNSPCs
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