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使用全血刺激试验的促炎性细胞因子IL -1β的精确和简单的测量
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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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使用全血刺激试验的促炎性细胞因子IL -1β的精确和简单的测量

DOI:
10.3791/2662-v

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06:29 min

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March 01, 2011

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, , ,
1Translational Immunology Section, Office of Science and Technology,National Institute of Arthritis and Musculoskeletal and Skin Diseases, 2Translational Autoinflammatory Disease Section, Office of the Clinical Director,National Institute of Arthritis and Musculoskeletal and Skin Diseases

Chapters

  • 00:05Title
  • 01:29Collection of Whole Blood
  • 02:02Preparation of Whole Blood
  • 03:53Stimulation of the Whole Blood
  • 05:15Cytokine Assay
  • 05:34Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个简单的免疫autoinflammatory表型的患者，来衡量生产促炎性细胞因子，如IL -1β生产。通过激活细胞与病原体相关分子模式，特别是与脂多糖，全血培养，细胞因子的分泌，可以方便地评估全血上清。

Tags

Pro-inflammatory CytokineIL-1βWhole Blood Stimulation AssayInflammatory ProcessesImmune CellsCytokine HomeostasisInnate Immune SystemPathogensDanger StimuliInterleukin-1 (IL-1)SecretionAutoinflammatory SyndromesLPSCellular Immune ResponsesPAMPsGram-negative Bacterial Endotoxin

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