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免疫印迹使用Invitrogen公司NuPage NOVEX双三MiniGels
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JoVE Journal
Biology
Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels
Please note that all translations are automatically generated.
Click here for the English version.
免疫印迹使用Invitrogen公司NuPage NOVEX双三MiniGels
DOI:
10.3791/264-v
•
22:45 min
•
August 22, 2007
•
Aubin Penna
,
Michael Cahalan
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:11
Introduction
00:59
Loading and Running an the SDS-PAGE Gel
08:12
Transferring Proteins to Nitrocellulose Membrane
15:34
Immunoblotting
20:22
Analyzing Western Blot
22:18
Conclusion
Summary
Automatic Translation
English (Original)
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这篇技术文章介绍了一个标准的程序,使用市售NuPAGE电泳迷你凝胶体系自Invitrogen Western印迹。
Tags
Western Blotting
Invitrogen NuPage Novex Bis Tris MiniGels
Immunoblotting
Protein Expression
Protein Analysis
Co-immunoprecipitation
Primary Antibody
Protein Separation
Molecular Weight
SDS-PAGE
Membrane Transfer
Chemiluminescence
Pre-cast Gels
User-friendly Equipment
NuPAGE Electrophoresis System
Laemmli Technique
Shelf Life
Molecular Weight Range
Acrylamide Percentage
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