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人类神经干细胞计数
JoVE Journal
Biology
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JoVE Journal
Biology
Counting Human Neural Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
人类神经干细胞计数
DOI:
10.3791/262-v
•
06:37 min
•
August 22, 2007
•
Steven Marchenko
,
Lisa Flanagan
1
Department of Pathology
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:12
Passaging Human Embryonic Neural Stem Cells
00:30
Preparing the Cell Suspension for Counting
03:54
Counting Cells in the Hemocytometer
Summary
Automatic Translation
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Automatic Translation
知识是需要许多组织培养操作活菌的确切人数。本协议描述了如何区分活细胞和死细胞和使用hemacytometer量化细胞。虽然它描述了人类神经干/前体细胞(hNSPCs)计数,它可用于其他类型的细胞。
Tags
Counting
Human Neural Stem Cells
Viable Cells
Cell Suspension
Immunocytochemistry
Cell Transfections
Hemacytometer
Live Cells
Dead Cells
Cell Concentration
Total Cell Number
Trypan Blue
Quadrants
Dilution Factor
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