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Neuroscience
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新皮質の器官型スライス標本からの全細胞記録
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Neuroscience
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JoVE Journal
Neuroscience
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
Please note that all translations are automatically generated.
Click here for the English version.
新皮質の器官型スライス標本からの全細胞記録
DOI:
10.3791/2600-v
•
10:29 min
•
June 03, 2011
•
Robert C. Foehring
,
Dongxu Guan
,
Tara Toleman
,
Angela R. Cantrell
1
Department of Anatomy and Neurobiology
,
University of Tennessee Health Science Center
Chapters
00:05
Title
01:02
Introduction
01:30
Preparations for Slicing
03:14
Slicing the Brain and Preparation for Culture
06:01
Transfecting the Slices Using a Gene Gun
07:31
Recording from Pyramidal Cells in Slices
09:10
Results: Recordings from Neocortical Pyramidal Cells in Organotypic Slice Culture
10:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
これは、錐体ニューロンから電気記録を作成する目的のための器官培養における新皮質スライス標本を準備し、維持するためのプロトコルです。
Tags
Whole Cell Recording
Organotypic Slice Preparation
Neocortex
Voltage-gated Potassium Channels
Pyramidal Neurons
Genetic Approach
Organotypic Culture Preparation
Laminar Pattern Of Cortex
Acute Neocortical Slices
Visually-identified Pyramidal Neurons
Infrared Illumination
Differential Interference Contrast Microscopy
Whole Cell Patch Clamp
Current-clamp
Voltage-clamp
Biolistic Transfection
Wild Type Potassium Channel DNA
Mutant Potassium Channel DNA
Function Study
Epifluorescence Microscopy
Green Fluorescent Protein (GFP)
Adjacent Untransfected Neurons
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