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使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
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Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
Please note that all translations are automatically generated.
Click here for the English version.
使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
DOI:
10.3791/2579-v
•
06:26 min
•
April 01, 2011
•
Askar M. Akimzhanov
,
Darren Boehning
1
Department of Neuroscience and Cell Biology
,
University of Texas Medical Branch
Chapters
00:05
Title
01:19
Pericam-mt Transfection and Cell Preparation
04:11
Image Processing and Analysis
04:37
Fluorescence Imaging Results
05:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了线粒体钙通量实时荧光成像测量方法。该方法采用一个基于循环permutated YFP的双激励比例的钙传感器选择性表达在线粒体(比例pericam吨)的优势。
Tags
Monitoring
Dynamic Changes
Mitochondrial Calcium Levels
Apoptosis
Genetically Encoded Calcium Sensor
Intracellular Calcium Concentration
Stimuli
Cellular Processes
Proliferation
Differentiation
Calcium Accumulation
Pro-apoptotic Factors
Cytosol
Living Cells
In Situ
High-resolution Fluorescent Imaging
Dual-excitation Ratiometric
Non-ratiometric Synthetic Calcium Indicator Dyes
Intracellular Calcium Signaling
Cytosolic Calcium Fluxes
Rhod-2
Rhod-FF
Synthetic Indicators
Mitochondria
Repetitive Increases
Mitochondrial Morphology
Leak Out Of Mitochondria
Long-term Experiments
Genetically Encoded Calcium Indicators
Green Fluorescent Protein (GFP)
Aequorin
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