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Journal
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Immunology and Infection
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用荧光素酶在小鼠细菌性感染的形象
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Using Luciferase to Image Bacterial Infections in Mice
Please note that all translations are automatically generated.
Click here for the English version.
用荧光素酶在小鼠细菌性感染的形象
DOI:
10.3791/2547-v
•
10:23 min
•
February 18, 2011
•
Mi Hee Chang
,
Suat L.G. Cirillo
,
Jeffrey D. Cirillo
1
Microbial & Molecular Pathogenesis
,
Texas A&M Health Science Center
Chapters
00:05
Title
01:30
Pulmonary Infection of Mice by Intratracheal Intubation
03:32
Preparation of Mice for Bioluminescence Imaging
04:24
Bioluminescence Imaging
05:56
Ex vivo Imaging and CFU Analysis for the Quantification of Bacteria in the Lungs
07:07
Analysis of Luminescence Imaging
08:53
Luminescent Imaging of Mice Intra-tracheally Infected with CBRluc-tagged Bacteria
09:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生物发光细菌感染在动物活体成像的方法进行了阐述。病原体被修改,以表达荧光素酶,使光全身显像在活的动物感染。荧光素酶表达的病原体可感染动物模型和疾病过程中产生的生物发光成像在实时可视化。
Tags
Luciferase
Imaging
Bacterial Infections
Mice
Bioluminescence Imaging
Real-time Monitoring
Cancer Cells
Stem Cells
Immune Cell Types
Viral Pathogens
Parasites
Conventional Strategies
Animal Models
Optical Imaging
Inoculation
Variability Between Animals
Reduce Animal Numbers
Data Collection
Recombinant Strains Of Bacteria Expressing Luciferase
Click Beetle Luciferase
Firefly Luciferase
Article
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