This content is Free Access.
Chapters
- 00:05Title
- 01:13Introduction
- 01:40Isolation of Cell Fractions from Buffy Coat
- 03:34NK Cell Expansion
- 06:34NK Cell Cytotoxicity Assay
- 08:33Representative Results
- 09:55Conclusion
Tags
ExpansionPurificationFunctional AssessmentHumanPeripheral BloodNK CellsNatural Killer CellsImmune SurveillanceInfectious MicroorganismsTumorsLimited AvailabilityIn VitroNK Cell ImmunotherapyMethodEfficiently ExpandVast QuantitiesFunctional NK CellsEx VivoK562 Cells Expressing Membrane-bound IL21Artificial Antigen-presenting Cell (aAPC)Cell ProductSteady-state LeukapheresisDonorDepletion Of T CellsPositive Selection Of NK CellsIL-2 Overnight ActivationSmall Numbers Of NK Cells Delivered In Clinical TrialsInability To Propagate NK Cells In VitroGenerating Sufficient Cell Numbers
