A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:22Introduction
- 00:53Preparation of Dissection Solution
- 02:15Adult Drosophila Selection
- 02:32Sex Differentiation
- 03:04Decapitation and Removal of Brain
- 04:19Removal of Trachea and Enzyme Treatment
- 05:27Stabilizing Brain in Recording Chamber
- 07:58Conclusion
Questo video mostra la procedura per isolare il cervello tutto da adulto Drosophila, in preparazione per la registrazione di singoli neuroni utilizzando tecnologia standard a cellula intera. Esso comprende le immagini di cellule GFP etichettati e neuroni visualizzati durante la registrazione.
Tags
Whole Cell RecordingsBrainAdult DrosophilaProcedureSingle NeuronsDissecting SolutionCaptureOptic LobesTracheaIsolated BrainNeuronsHandlingDamage PreventionHolderRecording ChamberElectrophysiology SetupGAL4 LineGFP ExpressionProjection Neurons (PNs)Live BrainNomarski ImageTargeted NeuronSpontaneous ActivityAction PotentialsSynaptic InputIn Situ Preparation
