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Journal
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Biology
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纯化的骨骼肌的先觉者
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Purification of Progenitors from Skeletal Muscle
Please note that all translations are automatically generated.
Click here for the English version.
纯化的骨骼肌的先觉者
DOI:
10.3791/2476-v
•
12:55 min
•
March 16, 2011
•
Lin Yi
,
Fabio Rossi
1
The Biomedical Research Centre
,
University of British Columbia
Chapters
00:05
Title
02:35
Tissue Collection
03:49
Dissociation of Tissue into Single Cells by Enzymatic Digestion
05:52
Antibody Staining and Sorting
08:45
Transplantation
09:45
Representative Myogenic and Adipogenic Progenitor Isolation and Transplantation Results
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酶解,表面标记和流式细胞仪检测纤维/成脂和小鼠骨骼肌生肌祖净化的方法。
Tags
Purification
Progenitors
Skeletal Muscle
Myogenic Progenitors
Satellite Cells
Basement Membrane
Vessel-associated Progenitors
Myofibroblasts
White Adipocytes
Proliferation
Trophic Support
Assays
Isolation
Transplantation
Flow Cytometry
Enzymatic Dissociation
Colony-forming Cells
Cell Types
Molecular Analysis
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