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Neuroscience
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建立小鼠胚胎神经干细胞培养神经球含量
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Neuroscience
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JoVE Journal
Neuroscience
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
Please note that all translations are automatically generated.
Click here for the English version.
建立小鼠胚胎神经干细胞培养神经球含量
DOI:
10.3791/2457-v
•
11:54 min
•
January 11, 2011
•
Hassan Azari
2
,
Sharareh Sharififar
,
Maryam Rahman
,
Saeed Ansari
,
Brent A. Reynolds
1
Department of Anatomical Sciences
,
Shiraz University of Medical Sciences, Shiraz, Iran
,
2
Department of Neurosurgery
,
The University of Florida
Chapters
00:01
Title
00:29
Introduction
00:57
Animal Dissection
02:40
E14 Brain Dissection
04:42
Tissue Dissociation
08:00
Passaging Neurospheres
10:10
Representative Results
11:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频协议演示应用程序的神经球实验胚胎每天14鼠脑的神经节金榜,神经干细胞的分离和扩张。
Tags
Embryonic Mouse Neural Stem Cell Culture
Neurosphere Assay
Stem Cells
Undifferentiated Cells
Cell Genesis
Aging Process
Injury And Disease
Neural Stem Cells (NSCs)
Serum-free Culture System
Isolation And Expansion Of NSCs
Embryonic CNS
In Vitro Expanded NSCs
CNS Cell Types
Reliable And Consistent Results
E14 Mouse Embryos
Brain Microdissection
Ganglionic Eminences
Single Cell Suspension
NSA Culture
Primary Neurospheres
Article
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