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成年鼠神经干细胞的分离和扩大使用神经干含量
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
DOI:

13:21 min

November 20, 2010

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Chapters

  • 00:00Title
  • 00:41Introduction
  • 01:04Animal Dissection
  • 02:25Brain Dissection
  • 04:31Tissue Preparation and Plating Cells
  • 09:04Passaging Neurospheres
  • 11:56Representative Results
  • 12:55Conclusion

Summary

Automatic Translation

这个视频协议演示,从成年小鼠脑室周围地区产生和扩大神经干细胞的神经球的含量测定方法,并提供技术见解,以确保可以实现重复性的神经球文化。

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