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Neuroscience
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成年鼠神经干细胞的分离和扩大使用神经干含量
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
Please note that all translations are automatically generated.
Click here for the English version.
成年鼠神经干细胞的分离和扩大使用神经干含量
DOI:
10.3791/2393-v
•
13:21 min
•
November 20, 2010
•
Hassan Azari
2
,
Maryam Rahman
,
Sharareh Sharififar
,
Brent A. Reynolds
1
Department of Anatomical Sciences
,
Shiraz University of Medical Sciences, Shiraz, Iran
,
2
Department of Neurosurgery
,
University of Florida
Chapters
00:00
Title
00:41
Introduction
01:04
Animal Dissection
02:25
Brain Dissection
04:31
Tissue Preparation and Plating Cells
09:04
Passaging Neurospheres
11:56
Representative Results
12:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频协议演示,从成年小鼠脑室周围地区产生和扩大神经干细胞的神经球的含量测定方法,并提供技术见解,以确保可以实现重复性的神经球文化。
Tags
Isolation
Expansion
Adult Mouse
Neural Stem Cells
Neurosphere Assay
Putative
Murine Brain
Reynolds And Weiss
Chemically Defined Serum-free Culture System
Growth Factor Responsive Precursor Cells
Epidermal Growth Factor
Basic Fibroblastic Growth Factor
Colonies
Undifferentiated Cells
Neurospheres
Subcultured
Pool Of Neural Stem Cells
Differentiate
Three Major Cell Types
CNS
Neurons
Astrocytes
Oligodendrocytes
Invaluable Tool
In Vitro Studies
Therapeutic Purposes
Video Demonstration
Periventricular Region
Reproducible Neurosphere Cultures
Article
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