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初级运动和感觉神经元上的静电聚- L -乳酸纳米纤维支架中定义的媒体文化
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
Please note that all translations are automatically generated.
Click here for the English version.
初级运动和感觉神经元上的静电聚- L -乳酸纳米纤维支架中定义的媒体文化
DOI:
10.3791/2389-v
•
16:03 min
•
February 15, 2011
•
Michelle K. Leach
,
Zhang-Qi Feng
,
Caitlyn C. Gertz
,
Samuel J. Tuck
,
Tara M. Regan
,
Youssef Naim
,
Andrea M. Vincent
,
Joseph M. Corey
3,4
1
Department of Biomedical Engineering
,
University of Michigan
,
2
State Key Laboratory of Bioelectronics
,
Southeast University
,
3
Department of Neurology
,
University of Michigan
,
4
Geriatric Research, Education and Clinical Center
,
Veterans Affairs Ann Arbor Health System
Chapters
00:05
Title
01:43
Preparation of Electrospun Fiber Substrates
04:40
Primary Cell Culture of Rat Embryonic Neuron
06:53
Motor Neuron (MN) Processing
09:29
Sensory Neuron (SN) Processing
10:56
Plating and Culture
12:41
Representative Results
14:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
不结盟纤维直接的神经元在体外的生长,是一个潜在的神经再生的支架组成部分。我们描述了一个准备静电纤维基材和主大鼠E15感觉(DRG)和运动神经元无血清培养的过程。用免疫细胞化学神经元的可视化也包括在内。
Tags
Culture
Primary Motor Neurons
Sensory Neurons
Defined Media
Electrospun Nanofiber Scaffolds
Electrospinning Technique
Alignment
Random Fibers
Biodegradable Polymers
Poly-L-lactic Acid (PLLA)
Nerve Regeneration
Primary Cell Culture System
Biomaterials
Dorsal Root Ganglia Explants
Dissociated Neurons
Article
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