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Journal
/
Immunology and Infection
/
铜绿假单胞菌
和
酿酒酵母
流细胞生物膜
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Pseudomonas aeruginosa
and
Saccharomyces cerevisiae
Biofilm in Flow Cells
Please note that all translations are automatically generated.
Click here for the English version.
铜绿假单胞菌
和
酿酒酵母
流细胞生物膜
DOI:
10.3791/2383-v
•
17:30 min
•
January 15, 2011
•
Martin Weiss Nielsen
,
Claus Sternberg
,
Søren Molin
,
Birgitte Regenberg
1
Department of Systems Biology
,
Danish Technical University
,
2
Department of Biology
,
University of Copenhagen
Chapters
00:00
Title
00:08
Introduction
00:33
Glueing of the Flow Cells
03:59
Assembly of Bubble Traps and Attachment of Tubings
05:22
Attachment of Flow Cells to the Bubble Traps
08:38
Sterilization of the System
10:07
Washing the System
11:26
Inoculating the Flow Cell
12:19
Incubation of the System
16:19
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
协议描述流通池系统中的应用激光共聚焦显微镜(CLSM)为不断发展和分析微生物生物膜。
Tags
Pseudomonas Aeruginosa
Saccharomyces Cerevisiae
Biofilm
Flow Cells
Microbial Communities
Altered Physiological Properties
Pathological Properties
Transparent Substratum
Non-destructive Examination
Real-time Studies
Flow Cell Model System
Reproducibility
Growth Chamber
Nutrients
Oxygen Supply
Peristaltic Pump
Waste Container
Continuous Supply Of Nutrients
Administration Of Antibiotics
Minimal Disturbance
Shear Stress
Bubble Trapping Device
Confocal Laser Scanning Microscopy (CLSM)
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