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Chapters
- 00:05Title
- 01:00Introduction
- 01:57Preparation and Isolation of Spider Abdomen
- 03:50Removal of Spider Abdominal Exoskeleton
- 04:53Dissection and Isolation of Silk-Producing Glands
- 07:42Evaluation of Gland mRNAs and Proteins Isolated through Spider Silk Gland Dissection
- 08:43Conclusion
Here we describe an efficient strategy to remove the silk-producing glands from the abdomen of female black widow spiders. This procedure allows the rapid isolation of the seven distinct silk-producing glands in a highly purified fashion, an important process for investigators studying spider silk production and fiber assembly.
Tags
MicrodissectionBlack Widow SpiderSilk-producing GlandsSilk FibersBiological FunctionsLocomotionPrey CaptureProtection Of OffspringFiber TypesMechanical PropertiesSilk-producing GlandsBiofactoriesSilk ProteinsBiochemical EventsLiquid To Solid ConversionMechanical StudiesSpider SilksTensile StrengthSteelStructure And FunctionProtein SequencesMolecular SignaturesSynthetic Silk Fibers
