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Journal
/
Immunology and Infection
/
前
肿瘤反应性T细胞
的体外
扩增Bryostatin 1/Ionomycin手段和共同的γ链细胞因子配方
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Ex vivo
Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
Please note that all translations are automatically generated.
Click here for the English version.
前
肿瘤反应性T细胞
的体外
扩增Bryostatin 1/Ionomycin手段和共同的γ链细胞因子配方
DOI:
10.3791/2381-v
•
07:20 min
•
January 14, 2011
•
Maciej Kmieciak
,
Amir Toor
,
Laura Graham
,
Harry D. Bear
,
Masoud H. Manjili
1
Department of Microbiology & Immunology
,
Virginia Commonwealth University- Massey Cancer Center
,
2
Department of Internal Medicine
,
Virginia Commonwealth University- Massey Cancer Center
,
3
Department of Surgery
,
Virginia Commonwealth University- Massey Cancer Center
Chapters
00:05
Title
00:55
Introduction
01:48
Mouse Model of Breast Cancer
02:14
Isolation of Lymphocytes
03:44
Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis
04:58
Determine Tumor-Specificity of the ex vivo Expanded T Cells
05:47
Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function
06:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为有效的协议<em>体外</em>描述从肿瘤引流淋巴结或其他淋巴组织的继发性肿瘤轴承主机的肿瘤反应性T细胞的扩张。该协议有选择地扩大肿瘤特异性T细胞过继免疫治疗乳腺癌。
Tags
Ex Vivo Expansion
Tumor-reactive T Cells
Bryostatin 1/Ionomycin
Common Gamma Chain Cytokines Formulation
Breast Cancer
Immune Responses
Adoptive Immunotherapy
Tumor-specific T Cells
Tumor Antigens
IL-2
IL-7
IL-15
IL-21
FVBN202 Transgenic Mouse Model
HER-2/neu Positive Breast Carcinoma
Adoptive T Cell Therapy
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