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一个孤立的视网膜制备,记录从基因标记的视网膜神经节细胞的光反应
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
Please note that all translations are automatically generated.
Click here for the English version.
一个孤立的视网膜制备,记录从基因标记的视网膜神经节细胞的光反应
DOI:
10.3791/2367-v
•
13:02 min
•
January 26, 2011
•
Tiffany M Schmidt
,
Paulo Kofuji
1
Department of Neuroscience
,
University of Minnesota
Chapters
00:05
Title
01:01
Introduction
01:33
Preparation of Solutions
02:29
Isolation of Retina from Mouse Eye
04:08
Treatment of Retina with Enzyme MIxture and Mounting in Recording Chamber
06:10
Localization of EGFP Expressing Ganglion Cells and Light Stimulation
09:51
Analysis of Anatomy of Recorded Ganglion Cells
11:21
Response of Retinal Ganglion Cell to Light Stimulation and Correlation with Morphology
12:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了一个描述如何从孤立在小鼠视网膜准备的解剖和记录。特别是,我们介绍了如何从一个荧光标记的神经节细胞的人口记录对光反应和随后查明并分析其形态。
Tags
Isolated Retinal Preparation
Light Response
Genetically Labeled Retinal Ganglion Cells
Vertebrate Vision
Rod And Cone Photoreceptors
ON And OFF Pathways
Bipolar Cells (BCs)
Retinal Ganglion Cells (RGCs)
Inner Plexiform Layer (IPL)
Excitatory Input
Illumination
Parallel Signaling
Brain Visual Nuclei
Fluorescent Reporter Mouse Lines
RGC Subtypes
Electrophysiological Recording
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