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Journal
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Biology
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适配器,网格蛋白相互作用
在体内
和
体外
研究
JoVE Journal
Biology
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JoVE Journal
Biology
In vivo
and
in vitro
Studies of Adaptor-clathrin Interaction
Please note that all translations are automatically generated.
Click here for the English version.
适配器,网格蛋白相互作用
在体内
和
体外
研究
DOI:
10.3791/2352-v
•
17:14 min
•
January 26, 2011
•
Daniel Feliciano
,
Jarred J. Bultema
,
Andrea L. Ambrosio
,
Santiago M. Di Pietro
1
Department of Biochemistry and Molecular Biology
,
Colorado State University
Chapters
01:34
Introduction
02:10
Generation of GFP-tagged Sla1 and Sla1 Clatherin-binding Mutant Yeast Strains
03:22
Fluorescence Microscopy
05:09
Fluorescence Image Analysis
06:41
Preparation of Yeast Cytosolic Extract
09:54
GST-fusion Protein Affinity Assay
11:53
Preparation of Total Cell Extract
13:36
Co-immunoprecipitation Assay
15:16
Representative Results
16:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
网格蛋白介导的内吞作用取决于适配器的蛋白质,协调货物选择和网格蛋白外衣大会。在这里,我们描述的程序来研究网格蛋白适配器的物理互动和活细胞成像的方法,作为一种模式使用酵母吞适配器蛋白Sla1p。
Tags
In Vivo
In Vitro
Adaptor-clathrin Interaction
Endocytic Pathway
Clathrin-coated Vesicles (CCVs)
Clathrin Lattice
Clathrin Triskelia
Clathrin-binding Adaptors
Membrane Proteins
Transmembrane Protein Cargo
CCV Formation Machinery
Clathrin Adaptors
N-terminal -propeller Domain
Modular Proteins
Linker Regions
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