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Immunology and Infection
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大脑和脊髓的单核细胞的分离,使用Percoll梯度
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
Please note that all translations are automatically generated.
Click here for the English version.
大脑和脊髓的单核细胞的分离,使用Percoll梯度
DOI:
10.3791/2348-v
•
09:58 min
•
February 02, 2011
•
Paula A. Pino
,
Astrid E. Cardona
1
Department of Biology and South Texas Center for Emerging Infectious Diseases
,
University of Texas at San Antonio – UTSA
Chapters
00:05
Title
01:27
Preparation of Reagents
02:09
Tissue Collection and Homogenization
02:56
Gradient Set Up
05:56
Antibody Staining
08:08
Representative Analysis by Flow Cytometry
09:29
Conclusion
Summary
Automatic Translation
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本文介绍一个快速的协议,有效地从大脑和脊髓组织,可以有效地利用流式细胞仪分析中分离出的单个核细胞。
Tags
Isolation
Brain
Spinal Cord
Mononuclear Cells
Percoll Gradients
Immune Cells
Central Nervous System
Infection
Trauma
Autoimmunity
Neurodegeneration
Phenotype
Effector Functions
Histochemical Approaches
Infiltrating Cells
CNS Pathology
In-situ Histochemistry
Immunofluorescent Staining Techniques
Antibodies
Immune Cell Subtypes
Histological Approaches
Flow Cytometry
Composition
Cellular Suspensions
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