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Immunology and Infection
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白三烯B实时成像
4
介导的细胞迁移和BLT1与β- arrestin的相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Real-time Imaging of Leukotriene B
4
Mediated Cell Migration and BLT1 Interactions with β-arrestin
Please note that all translations are automatically generated.
Click here for the English version.
白三烯B实时成像
4
介导的细胞迁移和BLT1与β- arrestin的相互作用
DOI:
10.3791/2315-v
•
13:45 min
•
December 23, 2010
•
Venkatakrishna R. Jala
,
Bodduluri Haribabu
1
Microbiology and Immunology, James Graham Brown Cancer Center
,
University of Louisville
Chapters
00:05
Title
01:13
Introduction
01:48
Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
02:42
Preparation of Lipid Loaded Micropipette
04:20
Live-cell Imaging of Cell Migration in Response to Ligand Application
05:25
Representative Results for Migration of BMDCs
06:03
Live Cell Imaging of Transfected RBL-2H3 Cells
09:49
Image Analysis
10:51
Representative Results
13:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍的方法,以确定具体的配体的白细胞趋化反应和识别细胞表面的受体,并用活细胞成像技术的胞质蛋白质之间的相互作用。
Tags
Leukotriene B4
Cell Migration
BLT1
β-arrestin
G-protein Coupled Receptors
Transmembrane Protein Family
Extracellular Signals
Intracellular Responses
Chemotaxis
Calcium Release
Gene Regulation
Ligand Binding
Conformational Changes
Heterotrimeric G-proteins
Second Messengers
Cyclic Adenosine Monophosphate (cAMP)
Inositol Triphosphate (IP3)
Diacyl Glycerol (DG)
Desensitization
Sequestration
Internalization
G-protein Receptor Kinases (GRKs)
Cytosolic Proteins
Membrane Translocation
Pro-inflammatory Lipid Molecule
Arachidonic Acid Pathway
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