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Chapters
- 00:00Title
- 02:36Transfection of HEK-293T with Recombinant Ion Channel cDNAs
- 04:52Plating Transfected HEK-293T Cells in Preparation for Electrophysiological Recording
- 07:36Electrophysiological Recording of Recombinant Channels in HEK-293T Cells
- 11:29Representative Imaging and Patch Clamp Recordings
- 12:13Conclusion
Tags
OptimizedTransfection StrategyExpressionElectrophysiological RecordingRecombinant Voltage-gated Ion ChannelsHEK-293T CellsIn VitroCultured Human Embryonic Kidney CellsGlass CoverslipsDensityElectrophysiological RecordingContactConfounding EffectsPlasma MembraneWhole-cell Patch Clamp RecordingDetectable CurrentsNoise LevelsIncubation PeriodsMembrane ExpressionCell-coverslip AdhesionMembrane StabilityTemperatureTransfected CellsHigh ConfluencyIon Channel Protein ExpressionGlass CoverslipsIncubation PeriodCell Attachment
