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末梢神経突起伸長のリアルタイムイメージングのためのGFP発現マウス胚の器官型スライス培養
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
Please note that all translations are automatically generated.
Click here for the English version.
末梢神経突起伸長のリアルタイムイメージングのためのGFP発現マウス胚の器官型スライス培養
DOI:
10.3791/2309-v
•
05:42 min
•
March 29, 2011
•
Isabel Brachmann
,
Kerry L. Tucker
1
Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology
,
University of Heidelberg
Chapters
00:05
Title
01:20
Preparation for Slicing and Culturing
02:08
Embryo Embedding
03:18
Slicing Procedure
04:23
Imaging Spinal Nerve Outgrowth
04:54
Representative Results of Imaging Spinal Nerve Outgrowth
05:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、末梢神経突起伸長の栽培とタイムラプスイメージングのための妊娠中期のマウス胚の器官型スライスを準備する方法を提示する。
Tags
Organotypic Slice Culture
GFP-expressing Mouse Embryos
Real-time Imaging
Peripheral Nerve Outgrowth
Ex Vivo Cultivation
Transgenic Mouse Line
TauGFP
Green Fluorescent Protein
Neurons
Central Nervous System
Peripheral Nervous System
Forelimb Innervation
Pharmacological Techniques
Genetic Techniques
Vibratome
Slice Preparation
Viability
Age-specific Development
Spinal Nerves
Spinal Cord
Dorsal Root Ganglia
Embryonic Day (E) E10 To E12 Slices
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