JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
マウスT細胞におけるT細胞受容体のレトロウイルス導入
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Please note that all translations are automatically generated.
Click here for the English version.
マウスT細胞におけるT細胞受容体のレトロウイルス導入
DOI:
10.3791/2307-v
•
10:14 min
•
October 22, 2010
•
Shi Zhong
,
Karolina Malecek
2
,
Arianne Perez-Garcia
,
Michelle Krogsgaard
1
NYU Cancer institute
,
New York University School of Medicine
,
2
Program in Structural Biology
,
New York University School of Medicine
Chapters
00:00
Title
01:37
Retroviral Transfection and T-cell Isolation
03:55
Prepapration of a Single-cell Suspension from Mouse Spleen
04:51
Isolation and Activation of Mouse T-cells
06:27
Infection of Activated T-cells
08:21
Expansion of Cells and Evaluation of Transduction Efficiency
08:55
Retroviral Expression of TCR on Mouse T-cells
09:32
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我々はレトロウイルス導入を用いて抗原特異的マウスT細胞を生成するためにプロトコルを提示
Tags
Retroviral Transduction
T-cell Receptors
Mouse T-cells
Immune System
Peptide Antigens
Antigen Presenting Cells
Functional Outcomes
Immunology Related Diseases
TCR Transgenic Mouse Models
Bone Marrow Chimera
Retroviral Transduction Method
Peptide-MHC Specificity
Downstream Applications
Human Immunotherapy
Adoptive Transfer
Cancer Treatment
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
初代ヒトナイーブおよびメモリーT細胞から誘導した調節性T細胞の生成
プライマリーヒトT細胞の
piggyBac
トランスポゾンシステムの変更
ナイーブCD4のアデノウイルス形質導入Tregの分化を研究するためにT細胞
T細胞の導出<em>体外</em>マウス胚性幹細胞からの
マウスナイーブCD4<sup> +</sup> T細胞の単離と<em>インビトロ</em> T細胞サブセットへの分化
骨髄前駆細胞のレトロウイルス形質導入は、T細胞受容体Retrogenicマウスを生成するために、
生成<em>デ・ノボ</em>センHemichainのレトロウイルス形質導入によって抗原特異的ヒトT細胞受容体
遺伝的分化を制御するメカニズムを研究するためのアプローチと機能などのヘルパーT細胞のレトロウイルス形質導入
腸内調節制御T細胞誘導の生体内増強
フローサイトメトリー解析のためのマウス腎臓レジデント
CD8+T
細胞の分離
Read Article