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Chapters
- 00:05Title
- 00:45Introduction
- 01:10Injecting mRNA into Oocytes
- 01:51Preparing the Perfusion System
- 04:26Preparing the Ag/AgCl Wire and the Data Acquisition Equipment
- 05:33Stripping the Vitelline Membrane from the Oocyte
- 06:14Preparing the Patch Pipettes
- 07:06Obtaining an Inside-Out Patch
- 08:08Patch Clamping
- 08:41Recording of Wild-Type BK Channels on and Oocyte Membrane Patch
- 09:57Conclusion
Ionische stroom van BK kanalen wordt geregistreerd met behulp van patch-clamp technieken. BK kanalen worden uitgedrukt in<em> Xenopus oöcyten</em> Door het injecteren van boodschapper-RNA. De intracellulaire oplossing tijdens de patch clamp opnames wordt bestuurd door een perfusie-systeem.
Tags
Patch ClampPerfusion TechniquesIon ChannelsXenopus OocytesBK ChannelsActivationStructure-function RelationshipNeurotransmitter ReceptorsSmooth Muscle ContractionHair Cell Frequency TuningNeurotransmitter ReleaseMembrane DepolarizationIntracellular Ca2+ And Mg2+Messenger RNA InjectionIncubationInside-out ConfigurationSingle Or Multiple ChannelsRecording CurrentsControlled Voltage
