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分析甲基化和其他 S腺苷 L同型半胱氨酸结合蛋白
JoVE Journal
Biology
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JoVE Journal Biology
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
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分析甲基化和其他 S腺苷 L同型半胱氨酸结合蛋白

DOI:
10.3791/2264-v

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17:12 min

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December 20, 2010

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, , , , , ,
1Department of Biochemistry / Analytics,caprotec bioanalytics GmbH, 2Institute of Organic Chemistry,RWTH Aachen University

Chapters

  • 00:05Title
  • 02:23Performing the Capture Assay and Controls
  • 08:26Tryptic Digest of Captured Proteins and Preparation of Peptides for LC-MS/MS
  • 09:50Peptide and Protein Identification via Automated Sequence Database Search
  • 12:33Representative Results of CCMS Experiments
  • 16:00Conclusion

Summary

Automatic Translation

Automatic Translation

捕获化合物三官能团的小分子功能可逆的光交联和净化的小分子蛋白质的相互作用，以减少蛋白质组的复杂性。在这里，我们使用捕捉化合物<em> S</em>腺苷<sub> L</sub>同型半胱氨酸具有约束力的选择性功能，从一个孤立的甲基化<em>大肠埃希氏菌</em>全细胞裂解液，并确定由MS。

Tags

MethyltransferasesS-adenosyl-L-homocysteine-binding ProteinsCapture Compound Mass SpectrometryAffinity ChromatographyActivity-based Protein ProfilingTrifunctional Capture CompoundsPhoto-crosslinkingSmall Molecule-protein InteractionsSelectivity FunctionSAHSorting FunctionBiotinSolid PhaseStreptavidin Magnetic BeadsOff-bead ConfigurationOn-bead ConfigurationSmall Molecule Of InterestSAMSAM-dependent MethyltransferasesDNA Methylation

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