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在体内 G蛋白偶联受体相互作用光谱分辨的双光子显微镜定量
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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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在体内 G蛋白偶联受体相互作用光谱分辨的双光子显微镜定量

DOI:
10.3791/2247-v

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14:26 min

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January 19, 2011

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, ,
1Department of Physics,University of Wisconsin – Milwaukee, 2Department of Biological Sciences,University of Wisconsin – Milwaukee

Chapters

  • 00:00Title
  • 01:46Introduction
  • 03:04Calibrating the Spectrally Resolved Two-photon Microscope
  • 08:58Collecting Data on Biological Samples of Interest
  • 12:36Protein Interactions Determined by Spectrally Resolved Two-photon Microscopy
  • 14:03Conclusion

Summary

Automatic Translation

Automatic Translation

通过采用一个光谱分辨的双光子显微成像系统，像素级别的地图福斯特共振能量转移（FRET）效率得到表达假设形成同源寡聚体复合物的膜受体细胞。从FRET的效率图，我们正在研究能够估计约低聚物复杂的化学计量信息。

Tags

In Vivo QuantificationG Protein Coupled Receptor InteractionsSpectrally Resolved Two-photon MicroscopyProtein InteractionsFRETFluorescent ProbesLiving CellsIndustrial ApplicationsBasic Biological KnowledgeDonor MoleculeAcceptor MoleculeLaser LightFluorescence EmissionSpectral PropertiesProtein Interactions DegreeCell ScanningMolecular CompositionAcquisition ProcessSpatial DeterminationQuantitative ValuesSpectrally Resolved Two-photon Microscope

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