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Visualisatie van UV-geïnduceerde Replicatie Intermediates in E. coli Met behulp van twee-dimensionale Agarose gel-analyse
JoVE Journal
Biology
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JoVE Journal Biology
Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
DOI:

10:36 min

December 21, 2010

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Chapters

  • 00:05Title
  • 00:57Introduction
  • 01:19Cell Growth and UV Irradiation
  • 03:14DNA Isolation
  • 05:502D Gel and Southern Analysis
  • 08:57Representative Results for Visualization of UV-induced Replication Intermediates in E. coli
  • 10:13Conclusion

Summary

Automatic Translation

We presenteren een procedure waarbij twee-dimensionale agarose-gel analyse kan worden gebruikt om de structuur van de replicatie tussenproducten die optreden na UV-straling te identificeren.

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