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分析的预微RNA和小分子RNA采用实时定量PCR(定量PCR)阵列
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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
Please note that all translations are automatically generated.
Click here for the English version.
分析的预微RNA和小分子RNA采用实时定量PCR(定量PCR)阵列
DOI:
10.3791/2210-v
•
10:58 min
•
December 03, 2010
•
Pauline Chugh*
1
,
Kristen Tamburro*
1
,
Dirk P Dittmer
1
Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research
,
University of North Carolina at Chapel Hill
Chapters
00:05
Title
01:21
Introduction
02:00
qPCR Reaction Sample Setup
03:03
Setup of the Pre-miRNA Assay Using the Freedom Tecan Evo Robot
05:17
Setup of the Pre-miRNA Assay Using the Matrix Electronic Multichannel Pipette
08:00
Representative Results for Profiling of Pre-micro RNAs and MicroRNAs using qPCR Arrays
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们将演示前的小分子RNA的设置和分析QPCR 96孔阵列以及使用机器人手用Thermo Scientific的矩阵多道移液器。
Tags
Profiling
Pre-micro RNAs
MicroRNAs
Quantitative Real-time PCR
QPCR Arrays
Gene Expression
Detection Limit
Input Amounts
Automated QPCR Setup
Reproducibility
High-throughput Experiments
Profiling Of Genes
Internal Plate Controls
Experimental Variables
Primer Pairs
Small Non-coding RNAs
Post-transcriptional Level Regulation
Primary MiRNA Transcript
Precursor MiRNA
Mature MiRNA Levels
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