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腺病毒介导的基因去除信号分子在原代培养的小鼠胚胎成纤维
JoVE Journal
Biology
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JoVE Journal Biology
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
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腺病毒介导的基因去除信号分子在原代培养的小鼠胚胎成纤维

DOI:
10.3791/2160-v

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11:00 min

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September 09, 2010

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, ,
1Department of Molecular and Cellular Biology,University of Guelph

Chapters

  • 00:00Title
  • 00:27Introduction
  • 02:59Materials & Methods
  • 09:36Conclusion

Summary

Automatic Translation

Automatic Translation

在这个视频中，我们使用一种腺病毒携带的Cre重组酶基因感染主要滑鼠胚胎成纤维细胞携带一个floxed Rac1的等位基因。

Tags

Adenovirus-mediatedGenetic RemovalSignaling MoleculesCultured Primary Mouse Embryonic FibroblastsCre-loxP SystemConditional DeletionCre RecombinaseFloxed DNARecombination EventExcisionAdenovirusCre Recombinase GeneInfectPrimary Mouse Embryonic Fibroblasts (MEFs)Floxed Rac1 AlleleMorphological ChangesActin CytoskeletonViral TransductionCre ExpressionStained Using Phalloidin DyeConfocal Laser Scanning Microscopy

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