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在调节Toll样受体激活Neu1唾液酸酶活动的检测
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
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在调节Toll样受体激活Neu1唾液酸酶活动的检测

DOI:
10.3791/2142-v

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09:04 min

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September 07, 2010

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, , , , , ,
1Department of Microbiology and Immunology,Queen’s University – Kingston, Ontario

Chapters

  • 00:00Title
  • 01:06Introduction
  • 01:48Resurrecting Frozen Macrophage Cells
  • 02:50Plating Cells for the Sialidase Assay
  • 04:42Assaying for Sialidase Activity
  • 06:50Sialidase Assay Results
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

唾液酸酶检测是一个简单的技术方法，将澄清新颖的TLR传感器，微生物感染和参与活巨噬细胞的细胞表面受体水平的炎症性疾病的分子机制（S）。

Tags

Neu1 Sialidase ActivityTOLL-like Receptor ActivationMammalian Toll-like ReceptorsPathogen-associated Molecular PatternsInfectious DiseasesInflammatory DiseasesAutoimmune DiseasesStructural Integrity Of Sensor ReceptorsLigand InteractionsSignaling ComponentsImmunological ProtectionDisease ModificationLigand-induced TLR ActivationNeu1 Sialidase ActivationNeurotrophin ReceptorsTrkATrkBMatrix Metalloproteinase-9 (MMP-9)Sialidase Assay

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