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Journal
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Biology
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Electroeluting DNA-Fragmenten
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Electroeluting DNA Fragments
Please note that all translations are automatically generated.
Click here for the English version.
Electroeluting DNA-Fragmenten
DOI:
10.3791/2136-v
•
06:13 min
•
September 05, 2010
•
Ana L. Zarzosa-Álvarez
,
Antonio Sandoval-Cabrera
,
Ana L. Torres-Huerta
,
Rosa Ma. Bermudez-Cruz
1
Department of Genetics and Molecular Biology
,
Research and Advanced Studies Center of National Polytechnic Institute
Chapters
00:00
Title
00:08
Introduction
00:53
Electrophoresis and Excision
01:44
Electroelution
02:57
Purification and Quantification
05:31
Summary
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
Dieses Verfahren ermöglicht die Aufreinigung von DNA-Fragmenten mit hoher Ausbeute.
Tags
Electroeluting DNA Fragments
Purified DNA Fragments
Molecular Biology
Electrophoresis
Agarose Gel
Acrylamide Gel
Binding And Elution
Glass Particles
Silica Particles
DEAE-cellulose Membranes
Crush And Soak Method
Electroelution
Commercial Purification Kits
Laboratory
Clean DNA
Sequencing
Radiolabeling
Enzymatic Restriction
Enzymatic Modification
Cloning
Sample Wells
Electroeluter
Current
Cushion Salt
Ethanol Precipitation
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