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短杆菌肽为基础的荧光分析法,确定小分子的修改脂质双层属性的潜在
JoVE Journal
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JoVE Journal
Biology
Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
Please note that all translations are automatically generated.
Click here for the English version.
短杆菌肽为基础的荧光分析法,确定小分子的修改脂质双层属性的潜在
DOI:
10.3791/2131-v
•
10:52 min
•
October 13, 2010
•
Helgi I. Ingólfsson
,
R. Lea Sanford
,
Ruchi Kapoor
,
Olaf S. Andersen
1
Department of Physiology and Biophysics
,
Weill Cornell Medical College
Chapters
00:00
Title
00:50
Introduction
01:22
Generation of 8-aminonaphthalene-1,3,6-trisulfonate (ANTS) Filled Liposomes
04:12
Fluorescent Solution Preparation
05:02
Fluorescence Instrument Setup
06:21
Performing a Gramicidin-based Fluorescence Assay
07:34
Data Analysis
09:16
Representative Results
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍一个快速荧光检测监控率作为衡量短杆菌肽通道活性的荧光猝灭。短杆菌肽通道,作为分子的力传感器监测双层跨越蛋白质检测中的脂质双层属性的变化。
Tags
Gramicidin-based Fluorescence Assay
Small Molecules
Lipid Bilayer Properties
Adsorption
Amphiphiles
Bilayer/solution Interface
Hydrophobic Interactions
Membrane Proteins
Protein Function Modulation
Off-target Drug Effects
Electrophysiological Assay
Linear Gramicidin Channels
Probes
Membrane Environment
Fluorescence Quenching
Fluorophore-loaded Large Unilamellar Vesicles
Quencher
Fluorescence Indicator/quencher Pair
8-aminonaphthalene-1
3
6-trisulfonate (ANTS)
Tl+
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