JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
纯净小鼠的B细胞的诱导和评估类开关重组
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Please note that all translations are automatically generated.
Click here for the English version.
纯净小鼠的B细胞的诱导和评估类开关重组
DOI:
10.3791/2130-v
•
09:49 min
•
August 13, 2010
•
Ahmad Zaheen
,
Alberto Martin
1
Department of Immunology
,
University of Toronto
Chapters
00:04
Title
00:12
Introduction
01:15
Isolation of Splenic B Cells
04:22
CFSE Cell Staining
05:47
Cell Stimulation with LPS
07:09
Flow Cytometry
08:45
Representative Results
09:32
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
抗原暴露后,激活的B细胞亚群接受类开关重组(CSR)的一个过程,产生不同的效应功能的抗体亚型。在本报告所述的协议解释CSR如何可诱发和分析<em>在体外</em>为研究B细胞功能的目的。
Tags
Induction
Assessment
Class Switch Recombination
Purified
Murine B Cells
Humoral Immunity
B Cell Activation
Immunoglobulin Locus
Genetic Modifications
Antibodies
IgM Antibody
IgG
IgA
IgE
Effector Functions
Immunity Maintenance
Diversification
Immunoglobulin Locus
Activation-induced Cytidine Deaminase (AID)
CSR Pathway
B Cell Function
Mouse Model
Spleen Isolation
Bacterial Lipopolysaccharide (LPS)
IgG3 Switching
Fluorescent Cell Staining Dye CFSE
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
激活的B细胞CD40的小鼠模型
在纯化造血干/祖细胞识别DNA突变
检测真正的IgE的表达小鼠B系细胞
诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠
人骨髓间充质干细胞(干细胞)的免疫调节性能评估
B组的小鼠模型<em>链球菌</em>阴道定植
粒子结合Bet v 1的变应原PM10的流式细胞分析
HDAC 抑制剂介导的 B 细胞 rna 和基因的基因组分析--诱导进行类开关 DNA 重组和血浆细胞分化
体外
和
活体
评估移植受体的 T、B 和髓细胞抑制活性和体液反应
小鼠边缘区 b 细胞剪切流诱导迁移的体外分析
Read Article