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在体内微循环在骨骼肌内重要的显微镜测量
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JoVE Journal Biology
In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy
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在体内微循环在骨骼肌内重要的显微镜测量

DOI:
10.3791/210-v

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07:31 min

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May 28, 2007

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, , , ,
1Department of Anesthesiology and Critical Care, Shriners Hospital for Children,Massachusetts General Hospital, and Harvard Medical School, 2Department of Geriatric Medicine, Graduate School of Medicine,The University of Tokyo

Chapters

  • 00:28Introduction
  • 02:09Mouse preparation
  • 03:08Red blood cell staining and injection
  • 04:49Discussion

Summary

Automatic Translation

Automatic Translation

提出一种新的微循环观察通用的方法。它被认为是适合长期观察，并结合pharmacophysiological或分子生物学干预。

Tags

In Vivo MicrocirculationSkeletal MuscleIntra-vital MicroscopyBlood Flow VelocityRed Blood Cell FluxMacroscopic ParametersImaging ModalitiesRBC VelocityRBC FluxPlasma Flow VolumeSpatial And Temporal HeterogeneityFlow RegulationMicrocirculatory RegulationIntravital MicroscopyJeff Lichtman’s MethodMouse Sternomastoid MusclesPKH26L-fluorescently Labeled RBCsWide-field MicroscopeMuscle ContractionElectrical StimulationRBC VelocityCapillary Density

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