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多色成像定时转基因斑马鱼:可视化由针对性的神经细胞消融激活视网膜干细胞
JoVE Journal
Biology
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JoVE Journal
Biology
Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation
Please note that all translations are automatically generated.
Click here for the English version.
多色成像定时转基因斑马鱼:可视化由针对性的神经细胞消融激活视网膜干细胞
DOI:
10.3791/2093-v
•
10:31 min
•
September 20, 2010
•
Junko Ariga*
1
,
Steven L. Walker*
1
,
Jeff S. Mumm
1
Department of Cellular Biology and Anatomy
,
Medical College of Georgia
Chapters
00:00
Title
00:47
Introduction
01:01
Selecting and Preparing Embryos/Larvae for Live Imaging
03:02
“Multicolor”
in vivo
Confocal Imaging
05:05
Spectral Separation Image Processing Using ImageJ Freeware
08:09
Representative Images of Neural Loss and Regeneration
10:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频,多色共焦成像时间推移和有针对性的细胞消融技术。定时成像是用来监测多种细胞类型的利益的行为<em>在体内</em>。有针对性的细胞消融有利于研究神经回路的功能和细胞特异性神经再生范例。
Tags
Multicolor Time-lapse Imaging
Transgenic Zebrafish
Retinal Stem Cells
Neuronal Cell Ablation
High-resolution Imaging
Living Zebrafish Larvae
Fluorescent Reporters
Cellular Interactions
Experimental Manipulations
Serial Imaging
Fluorophores
Hypopigmented Mutant Lines
Membrane Targeted Fluorescent Reporters
Morphological Detail
Cellular Details
Chemically-induced Ablation
Nitroreductase Mediated Ablation
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