JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
髓母细胞瘤干细胞的分离,浓缩,和维修
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
髓母细胞瘤干细胞的分离,浓缩,和维修
DOI:
10.3791/2086-v
•
06:32 min
•
September 01, 2010
•
Xi Huang
,
Tatiana Ketova
,
Ying LItingtung
,
Chin Chiang
1
Department of Cell and Developmental Biology
,
Vanderbilt University
Chapters
00:00
Title
00:59
Introduction
01:20
Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
03:34
Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
04:26
Immunofluorescent Staining of Cultured Medulloblastoma Cells
04:53
Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
06:17
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个协议描述和异位刺猬通路活性的突变小鼠的髓母细胞瘤肿瘤干细胞的分离,浓缩,维护。
Tags
Isolation
Enrichment
Maintenance
Medulloblastoma Stem Cells
Brain Tumors
Neurosphere Assays
Neural Stem Cells
Tumorigenesis
Tumor Progression
Shh Signaling
Cerebellar Development
Cancer Stem Cells
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
在免疫功能低下小鼠颅内髓母细胞瘤细胞原位Allografting
成年鼠神经干细胞的分离和扩大使用神经干含量
在完整的感觉器官前体细胞的活细胞成像
果蝇
蛹
从人类毛囊神经嵴干细胞的分离和培养的
神经母细胞含量:从区分神经干细胞后代使用流式细胞仪,神经祖细胞的生成和富集含量
从小鼠胚胎神经管的神经嵴细胞的分离和培养
原发性脑多巴胺能神经元的分离,培养和长期维持胚胎脑鼠类
分离和成人神经干细胞的培养从鼠标Subcallosal区
胚胎小鼠神经干细胞的分离与培养
通过Sialoglycan的代谢标记识别主要神经干细胞和祖细胞的细胞表面标记
Read Article